Русский
Association of polymorphic markers in the genes of congenital immunity receptors with the development and severity of COVID-19 clinical course
DOI: https://dx.doi.org/10.18565/therapy.2023.2.14-20
Kalyuzhnaya N.O., Meremyanina E.A., Abramova N.D., Malinnikova E.Yu., Safonova A.B., Chuchalin A.G., Svitich O.A.
1) I.I. Mechnikov Scientific Research Institute of Vaccines and Serums of the Ministry of Education and Science of Russia, Moscow; 2) Russian Medical Academy of Continuous Professional Education of the Ministry of Healthcare of Russia, Moscow; 3) N.I. Pirogov Russian National Research Medical University of the Ministry of Healthcare of Russia, Moscow; 4) I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University); 5) N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Healthcare of Russia, Moscow
The aim of the study is to search and analyze the associations of polymorphic markers rs2075822 in the NOD1 gene, rs3135499 and rs8057341 in the NOD2 gene, as well as rs1990760 in the IFIH1 gene with the development and severity of COVID-19 disease.
Materials and methods. To perform the study, venous blood was taken from patients with different degrees of severity of the course of COVID-19, as well as from healthy individuals. DNA was isolated from the obtained samples, after which DNA was analyzed using the real-time polymerase chain reaction (RT-PCR) method for selected polymorphic markers.
Results. We found that markers rs3135499 and rs8057341 are associated with the incidence of coronavirus infection. Moreover, markers rs1990760 and rs2075822 are associated with a mild course of infection, while rs8057341 is associated with a course of moderate severity.
Conclusion. Obtained could be used as predictive markers in prognosticating the severity of the course of coronavirus infection in healthy individuals with an unfavorable anamnesis and a tendency to pulmonary diseases, as well as for early detection of patients predisposed to a severe course of COVID-19 among all patients with already developed clinical manifestations of the disease.
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About the Autors
Natalia O. Kalyuzhnaya, junior researcher at the Laboratory of molecular immunology, I.I. Mechnikov Scientific Research Institute of Vaccines and Serums of the Ministry of Education and Science of Russia. Address: 115088, Moscow, 15 1st Dubrovskaya Str. E-mail: nat_kalyuzhnaya@mail.ru. ORCID: https://orcid.org/0000-0002-1668-1846
Ekaterina A. Meremyanina, PhD in Medical Sciences, researcher at the Laboratory of molecular immunology, I.I. Mechnikov Scientific Research Institute of Vaccines and Serums of the Ministry of Education and Science of Russia, senior lecturer at the Department of virology, Russian Medical Academy of Continuous Professional Education of the Ministry of Healthcare of Russia. Address: 115088, Moscow, 15 1st Dubrovskaya Str. E-mail: ekaterina@meremianina.ru. ORCID: https://orcid.org/0000-0003-4334-1473
Natalya D. Abramova, junior researcher at the Laboratory of molecular immunology, I.I. Mechnikov Scientific Research Institute of Vaccines and Serums of the Ministry of Education and Science of Russia. Address: 115088, Moscow, 15 1st Dubrovskaya Str. E-mail: and960911@gmail.com. ORCID: https://orcid.org/0000-0002-7307-0515
Elena Yu. Malinnikova, MD, associate professor, head of the Department of virology, Russian Medical Academy of Continuous Professional Education of the Ministry of Healthcare of Russia. Address: 125445, Moscow, 19/38 Belomorskaya Str. E-mail: malinacgb@mail.ru. ORCID: https://orcid.org/0000-0002-5501-5707
Anna B. Safonova, resident of N.N. Blokhin National Medical Research Center of Oncology of the Ministry of Healthcare of Russia. Address: 115478, Moscow, 24 Kashirskoe Lane. E-mail: apotapova97@mail.ru,
ORCID https://orcid.org/0009-0000-6927-1966
Alexander G. Chuchalin, MD, professor, academician of RAS, head of the Department of hospital therapy of the Faculty of pediatrics, N.I. Pirogov Russian National Research Medical University of the Ministry of Healthcare of Russia, Chairman of the Board of the Russian Respiratory Society. Address: 105077, Moscow, 32/4 11st Parkovaya Str. E-mail: pulmomoskva@mail.ru. ORCID: https://orcid.org/0000-0002-6808-5528
Oksana A. Svitich, MD, professor of RAS, corresponding member of RAS, professor of the Department of microbiology, virology and immunology named after academician A.A. Vorobyov of F.F. Erisman Institute of Public Health of I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University), director of I.I. Mechnikov Scientific Research Institute of Vaccines and Serums of the Ministry of Education and Science of Russia. Address: 105064, Moscow, 5A Maly Kazenny Lane. E-mail: svitichoa@yandex.ru.
ORCID: https://orcid.org/0000-0003-1757-8389
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