Metagenomic analysis of oropharyngeal microbiota in patients with different phenotypes of chronic obstructive pulmonary disease


M.A. Karnaushkina, S.V. Fedosenko, V.A. Petrov, A.G. Malyavin, A.D. Strutynskaya, N.A. Cherkasova, L.M. Ogorodova

1 I.M. Sechenov First Moscow state medical university, Moscow 2 Siberian state medical university, Tomsk 3 A.I. Yevdokimov Moscow state university of medicine and dentistry, Moscow 4 N.I. Pirogov Russian national research medical university, Moscow
Backgrоund. Chronic obstructive pulmonary disease (COPD) is characterized by progressive limitation of airflow rate of the respiratory tract to noxious particles. It is shown that FEV1, which characterizes the speed limit airflow, not allows with high probability to predict the rate of progression of COPD. Aims. To characterize the features of the taxonomic composition ща oropharyngeal microbiota in patients with COPD. Materials and methods. The study included 98 patients with COPD. On each of 2 visits, according to the study protocol, all patients were conducted clinical and functional examination. In addition, at the first visit, during stable period of disease, smears were collected. The taxonomic identification of microbiota was carried out using sequence analysis of conservative sites of bacterial 16S pRNA. To determine the taxonomic differences between groups of samples a method based on linear regression analysis, adjusted for metagenomic data, was applied. For assessment of the trends in changing the microbiota under influence of frequency of exacerbations and dyspnea generalized linear model was used. Results. The results of the study demonstrated a number of significant differences in the ratio of its various microbes, depending on the clinical and functional characteristics of COPD, including the frequency of exacerbations, dyspnea and COPD phenotype.
Keywords: oropharyngeal microbiota, forced expiratory volume in 1 second, сhronic obstructive pulmonary disease, phenotypes, exacerbation
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About the Autors

Maria A. Karnaushkina, PhD, associate professor of the Department of hospital therapy №2, I.M. Sechenov First Moscow state medical university. Address: 119991, Moscow, 8/2 Trubetskaya St. Tel.: +79162009374.
E-mail: kar3745@yandex.ru

Sergey V. Fedosenko, MD, assistant of the Department of general practice and outpatient therapy, Siberian state medical university. Address: 634050, Tomsk, 2 Moskovsky trakt. Tel.: +79138102311. E-mail: s-fedosenko@mail.ru

Vyacheslav A. Petrov, junior researcher of Central research laboratory Siberian state medical university». Address: 634050, Tomsk, 2 Moskovsky trakt . Tel.: +79234018848. E-mail: vyacheslav.a.petrov@mail.ru

Andrey G. Malyavin, MD, professor, Department of phthisiology and pulmonology, A.I. Yevdokimov Moscow State University of medicine and dentistry. Address: 127473, Moscow, 20/1 Delegatskaya St. Tel.: +79165478373. E-mail: maliavin@mail.ru

Anastasiya D. Strutynskaya, student of pediatric faculty, 5 course, N.I. Pirogov Russian national research medical university. Address: 117997, Moscow, 1 Ostrovityanova St. Tel: +79096857538. E-mail: strutynskaya@yandex.ru

Nataliya A. Cherkasova, PhD, associate professor of the Department of hospital therapy №2, I.M. Sechenov First Moscow state medical university. Address: 119991, Moscow, 8/2 Trubetskaya St. Tel.: +79166817166.
E-mail: cherkasova31@gmail.com

Lyudmila M. Ogorodova, MD, professor, corresponding member of RAS, deputy minister of education and science of the Russian Federation, head of the Department of pediatrics faculty with the course of children diseases, medical faculty, Siberian state medical university. Address: 634050, Tomsk, 2 Moskovsky trakt. Tel.: +79689026783. E-mail: lm-ogorodova@mail.ru


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