The role of innate immune factors in acute myeloid leukemia before and after chemotherapy


DOI: https://dx.doi.org/10.18565/therapy.2021.4.41-47

Vinnitskaya A.B., Svitich O.A., Golenkov A.K., Klinushkina E.F., Zaitseva T.A., Davydova N.V., Zverev V.V.

1) I.I. Mechnikov Scientific Research Institute of Vaccines and Serums, Moscow; 2) I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University); 3) M.F. Vladimirsky Moscow Regional Research Clinical Institute
Abstract. The aim was to study the effect of chemotherapy on chemotaxis, as well as the expression of CXCL12, EGFR, CCR4 genes in mononuclear cells (MNCs) from patients with acute myeloid leukemia (AML) after CXCL12 exposure.
Material and methods. Blood isolated from AML patients, as well as blood from healthy donors served as a clinical material. The study of chemotaxis was carried out using a Boyden chamber. CXCL12 was used as a chemoattractant. Gene expression was studied by means of using real-time polymerase chain reaction (RT-PCR).
Results. Decreased chemotaxis of MNCs isolated from AML patients before chemotherapy started, in the direction towards CXCL12 was determined. An increase in the expression of the heterologous receptor CCR4 gene and a decrease in the expression of the EGFR gene before chemotherapy under the influence of CXCL12 were shown. After the therapy, the expression profiles change their direction.
Conclusion. Above-described studies and obtained data demonstrate that there are existing other non-classical pathways of chemotaxis activation, as well as the possibility of cross-activation of heterologous receptors.
Keywords: Boyden’s chamber, acute myeloid leukemia, chemotaxis, CCR4, CXCL12, EGFR, chemokines
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About the Autors

Alexandra B. Vinnitskaya, PhD, junior researcher of I.I. Mechnikov Scientific Research Institute of Vaccines and Serums. Address: 105064, Moscow, 5A Maly Kazenny Lane. Tel.: +7 (909) 691-51-57. E-mail: byzonka@yandex.ru
Oksana A. Svitich, MD, professor, corresponding member of RAS, professor of the Department of microbiology, virology and immunology of F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University), Director of I.I. Mechnikov Scientific Research Institute of Vaccines and Serums. Address: 105064, Moscow, 5A Maly Kazenny Lane. Tel.: +7 (926) 148-83-22. E-mail: svitichoa@yandex.ru. ORCID: 0000-0003-1757-8389
Anatoly K. Golenkov, MD, professor, head of the Clinic of clinical hematology and immunotherapy, M.F. Vladimirsky Moscow Regional Research Clinical Institute. Address: 129110, Moscow, 61/2 Shchepkina Str. Tel.: +7 (495) 631-05-73. E-mail: golenkov@monikiweb.ru
Elena F. Klinushkina, head of the Department of clinical hematology and immunotherapy, M.F. Vladimirsky Moscow Regional Research Clinical Institute. Address: 129110, Moscow, 61/2 Shchepkina Str.
Tatyana A. Zaitseva, PhD, senior lecturer of the Department of microbiology, virology and immunology of F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University). Address: 25009, Moscow, 11/10 Mokhovaya Str. E-mail: zaytseva_t_a@staff.sechenov.ru
Natalya V. Davydova, PhD, associate professor of the Department of microbiology, virology and immunology of F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University). Address: 25009, Moscow, 11/10 Mokhovaya Str. E-mail: kaf-microb-pmgmu@yandex.ru
Vitaly V. Zverev, doctor of biological sciences, professor, academician of RAS, head of the Department of microbiology, virology and immunology of F.F. Erisman Institute of Public Health, I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of Russia (Sechenov University), scientific director of I.I. Mechnikov Scientific Research Institute of Vaccines and Serums. Address: 105064, Moscow, 5A Maly Kazenny Lane. E-mail: zverev_v_v@staff.sechenov.ru. ORCID: 0000-0002-0017-1892


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