Study of colon microbiological culture in patients considering themselves to be healthy


DOI: https://dx.doi.org/10.18565/therapy.2023.10.68-73

Krylova I.A., Lyamin A.V.

Samara State Medical University of the Ministry of Healthcare of Russia
Abstract. Detection of chronic non-infectious diseases (CNIDs) as early as possible is one of the priority tasks of the world healthcare services. A factorial prevention strategy is considered to be the basis, at which the general conditions for chronic CNIDs development are determined. Influence of quantitative and qualitative composition of the intestinal microbiota at occurrence and supporting of chronic diseases has been proven, but its influence is not taken into account in modern programs for prevention and prophylactic medical examination service.
The aim: to determine the species diversity of colonic luminal microbiotic culture in outpatients who consider themselves to be healthy.
Material and methods. Survey, examination, anthropometry assessment, collection of clinical and laboratory data of outpatients (n=114) aged from 18 to 72 years were carried out. Species composition of the luminal intestinal microbiota was analyzed with the identification of all grown microorganisms using MALDI-ToF mass spectrometry methodic.
Results. About 50% of patients who considered themselves healthy and did not apply to a doctor had elevated body mass index values. The composition of the luminal intestinal microbiota showed a tendency towards a decrease in bacterization in general and a decrease in the content of bifidobacteria with increasing of patients’ age. Normal body weight of patients was found in case of the presence of bacteroids and lactobacilli in luminalis microbiota. The dependence of patient’s well-being on the species diversity of the present luminal microbiota was found.
Conclusion. Basing on the results of the study, it could be assumed that the determination of the colonal luminal microbiota as a part of prevention and clinical examination programs can provide additional important information about the risk factors for CNIDs presence in patients.
Keywords: early detection of diseases, chronic non-infectious diseases, intestinal microbiota
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About the Autors

Irina A. Krylova, PhD in Medical Sciences, associate professor, associate professor of the Department of family medicine with a course in telemedicine technologies, Samara State Medical University of the Ministry of Healthcare of Russia. Address: 443099, Samara, 45 Leningradskaya St.
E-mail: raznoe.2009@list.ru
ORCID: https://orcid.org/0000-0003-1757-0774
Artem V. Lyamin, MD, professor, professor of the Department of general and clinical microbiology, immunology and allergology, director of the Scientific and Educational Professional Center for Genetic and Laboratory Technologies, Samara State Medical University of the Ministry of Healthcare of Russia. Address: 443079, Samara, 18 Gagarina St.
E-mail:avlyamin@mail.ru
ORCID: https://orcid.org/0000-0002-5905-1895


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