Русский
Study of colon microbiological culture in patients considering themselves to be healthy
DOI: https://dx.doi.org/10.18565/therapy.2023.10.68-73
Krylova I.A., Lyamin A.V.
Samara State Medical University of the Ministry of Healthcare of Russia
The aim: to determine the species diversity of colonic luminal microbiotic culture in outpatients who consider themselves to be healthy.
Material and methods. Survey, examination, anthropometry assessment, collection of clinical and laboratory data of outpatients (n=114) aged from 18 to 72 years were carried out. Species composition of the luminal intestinal microbiota was analyzed with the identification of all grown microorganisms using MALDI-ToF mass spectrometry methodic.
Results. About 50% of patients who considered themselves healthy and did not apply to a doctor had elevated body mass index values. The composition of the luminal intestinal microbiota showed a tendency towards a decrease in bacterization in general and a decrease in the content of bifidobacteria with increasing of patients’ age. Normal body weight of patients was found in case of the presence of bacteroids and lactobacilli in luminalis microbiota. The dependence of patient’s well-being on the species diversity of the present luminal microbiota was found.
Conclusion. Basing on the results of the study, it could be assumed that the determination of the colonal luminal microbiota as a part of prevention and clinical examination programs can provide additional important information about the risk factors for CNIDs presence in patients.
Literature
1. Budreviciute A., Damiati S., Sabir D.K. et al. Management and prevention strategies for non-communicable diseases (NCDs) and their risk factors. Front Public Health. 2020; 8: 574111. https://dx.doi.org/10.3389/fpubh.2020.574111. 2. Приказ Минздрава России (Министерство здравоохранения РФ) от 27 апреля 2021 г. № 404н «Об утверждении Порядка проведения профилактического медицинского осмотра и диспансеризации определенных групп взрослого населения». Доступ: https://www.garant.ru/products/ipo/prime/doc/401314440/ (дата обращения – 01.12.2023). [Order of the Ministry of Healthcare of Russia (Ministry of Healthcare of the Russian Federation) dated April 27, 2021 No. 404n «On approval of the Procedure for conducting a preventive medical examination and medical examination of certain groups of the adult population». URL:https://www.garant.ru/products/ipo/prime/doc/401314440/ (date of access – 01.12.2023) (In Russ.)]. 3. Глобальные факторы риска для здоровья. ВОЗ. Женева. 2015; 70 с. Доступ: https://apps.who.int/iris/bitstream/handle/10665/44203/9789244563878_rus.pdf;jsessi (дата обращения – 01.12.2023). [Global health risk factors. WHO. Geneva. 2015; 70 pp. URL: https://apps.who.int/iris/bitstream/handle/10665/44203/9789244563878_rus.pdf;jsessi (date of access – 01.12.2023) (In Russ.)]. 4. Баланова Ю.А., Имаева А.Э., Концевая А.В. с соавт. Эпидемиологический мониторинг факторов риска хронических неинфекционных заболеваний в практическом здравоохранении на региональном уровне. Методические рекомендации под редакцией С.А. Бойцова. М. 2016; 111 с. Доступ: https://gnicpm.ru/wp-content/uploads/2020/01/metod_rek_epidmonitoring.pdf (дата обращения – 01.12.2023). [Balanova Yu.A., Imaeva A.E., Kontsevaya A.V. et al. Epidemiological monitoring of risk factors for chronic non-communicable diseases in practical healthcare at the regional level. Guidelines edited by S.A. Boytsov. Moscow. 2016; 111 pp. URL: https://gnicpm.ru/wp-content/uploads/2020/01/metod_rek_epidmonitoring.pdf (date of access – 01.12.2023) (In Russ.)]. 5. Задворная О.Л., Борисов К.Н. Факторы риска неинфекционных заболеваний. Учебное пособие. М.: ФГБОУ ДПО РМАНПО. 2017; 65 с. [Zadvornaya O.L., Borisov K.N. Risk factors for non-communicable diseases. Textbook. Moscow: Russian Medical Academy of Continuous Professional Education. 2017; 65 pp. (In Russ.)]. ISBN: 978-5-7249-2739-0. 6. Nicoletti A., Ponziani F.R., Biolato M. et al. Intestinal permeability in the pathogenesis of liver damage: From non-alcoholic fatty liver disease to liver transplantation. World J Gastroenterol. 2019; 25(33): 4814–34. https://dx.doi.org/10.3748/wjg.v25.i33.4814. 7. Ulluwishewa D., Anderson R., McNabb W. Regulashion of tight junction permeability by intestinal bacteria and dietary components.J Nutr. 2011; 141(5): 769–76. https://dx.doi.org/10.3945/jn.110.135657. 8. Zou Y., Lin X., Xue W. et al. Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces. Sci Rep. 2021; 11(1): 11340. https://dx.doi.org/10.1038/s41598-021-90786-3. 9. Buffie C.G., Bucci V., Stein R.R. et al. Precision microbiome reconstitution restores bile acid mediated resistance to Clostridium difficile. Nature. 2015; 517(7533): 205–8. https://dx.doi.org/10.1038/ nature13828. 10. Assimakopoulos S.F., Triantos C., Maroulis I., Gogos C. The role of the gut barrier function in health and disease. Gastroenterology Res. 2018; 11(4): 261–63. https://dx.doi.org/10.14740/gr1053w. 11. Alvarez J., Real J.M.F., Guarner F. et al. Gut microbes and health. Gastroenterol Hepatol. 2021; 44(7): 519–35.https://dx.doi.org/10.1016/j.gastre.2021.01.002.
About the Autors
Irina A. Krylova, PhD in Medical Sciences, associate professor, associate professor of the Department of family medicine with a course in telemedicine technologies, Samara State Medical University of the Ministry of Healthcare of Russia. Address: 443099, Samara, 45 Leningradskaya St.
E-mail: raznoe.2009@list.ru
ORCID: https://orcid.org/0000-0003-1757-0774
Artem V. Lyamin, MD, professor, professor of the Department of general and clinical microbiology, immunology and allergology, director of the Scientific and Educational Professional Center for Genetic and Laboratory Technologies, Samara State Medical University of the Ministry of Healthcare of Russia. Address: 443079, Samara, 18 Gagarina St.
E-mail:avlyamin@mail.ru
ORCID: https://orcid.org/0000-0002-5905-1895
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