Role of microRNA in multifactorial connective tissue pathology


DOI: https://dx.doi.org/10.18565/therapy.2020.6.35-39

Yalaev B.I., Tyurin A.V., Nurlygayanov R.Z., Khusainova R.I.

1) Institute of biochemistry and genetics of Ufa Federal Research Center of the Russian Academy of Sciences; 2) Bashkir State Medical University of the Ministry of Healthcare of Russia, Ufa; 3) City clinical hospital No. 21, Ufa
The aim of the research was to study the role of polymorphic variants of microRNA binding sites in key genes of structural proteins and bone tissue homeostasis in the generation of a low level of bone mineral density (BMD) and the osteoporotic fractures occurence in males and females from the Volga–Ural region of Russia.
Material and methods. The polymorphic variants of the binding sites rs1061237 (c. *88T> C), rs9659030 (c. *1183A> G), rs11540149 (c. *1865G> A), rs6854081 (c. *3156T> C), rs1042673 (c. * 811A> G), rs10793442 (c. *332G> T), rs10098470 (c. *1073C> T), rs1054204 (c. *582G> C) and rs1042840 (c. * 949A> G) using DNA sample genotyping techniques in a sample of postmenopausal women and men over 45 years old were studied.
Results. There were fixed significant associations of the * A allele of the rs11540149 polymorphic locus (c. * 1865G> A) of the VDR gene with osteoporotic fractures and low bone mineral density in the general sample of males, * G * G genotype of the rs6854081 locus of the FGF2 gene with fractures in the general sample of females and females of Tatar ethnicity, allele * T of locus rs10098470 of TPD52 gene with fractures in females of Russian ethnicity, allele * A of locus rs10793442 (c. * 332G> T) of gene ZNF239 with osteoporosis and osteopenia in the general sample of females and genotype * G * G locus rs1054204 of the SPARC gene with fractures and a low level of BMD in the total sample of males with fractures in the total sample of females and females of Tatar ethnicity.
Conclusion. A number of associations demonstrating the significant role of epigenetic factors in the formation of primary osteoporosis in men and women has been identified.
Keywords: microRNA binding site, epigenetics, osteopenia, bone mineral density, osteoporosis
Read entire article

Literature


  1. Chiarelli N., Carini G., Zoppi N. et al. Transcriptome-wide expression profiling in skin fibroblasts of patients with joint hypermobility syndrome/Ehlers–Danlos syndrome hypermobility type. PLoS One. 2016; 11(8): e0161347. doi: 10.1371/journal.pone.0161347.

  2. Гребенникова Т.А., Белая Ж.Е., Рожинская Л.Я. с соавт. Эпигенетические аспекты остеопороза. Вестник РАМН. 2015; 5: 541–548. [Grebennikova T.A., Belaya Zh.E., Rozhinskaya L.Ya. et al. Epigenetic aspects of osteoporosis. Vestnik RAMN. 2015; 5: 541–548 (In Russ.)]. doi: 10.15690/vramn.v70.i5.1440.

  3. Bernabei R., Martone A.M., Ortolani E. et al. Screening, diagnosis and treatment of osteoporosis: a brief review. Clin Cases Miner Bone Metab. 2014; 11(3): 201–07.

  4. Капланова З.А., Шамов И.М., Сутаева Т.Р. Стоматологические пациенты и факторы риска остеопороза у них. Известия Дагестанского государственного педагогического университета. 2015; 2: 83–85. [Kaplanova Z.A., Shamov I.M., Sutaeva T.R. Dental patients and their risk factors for osteoporosis. Izvestiya Dagestanskogo pedagogicheskogo universiteta. 2015; 2: 83–85 (In Russ.)].

  5. Безлюдная Н.В. Актуальность проблемы остеопороза. ФГБУ «Федеральный центр травматологии, ортопедии и эндопротезирования» Минздрава России. [Электронный ресурс]. URL: http://www.orthoscheb.com/upload/1.pdf (дата обращения – 05.08.2019). [Bezlyudnaya N.V. The urgency of the problem of osteoporosis. Federal Center for traumatology, orthopedics and endoprosthetics of the Ministry of Healthcare of Russia. [Electronic resource]. URL: http://www.orthoscheb.com/upload/1.pdf (date of access – 05.08.2019) (In Russ.)].

  6. Chen Y.-J., Chang W.-A., Huang M.-S. et al. Identification of novel genes in aging osteoblasts using next-generation sequencing and bioinformatics. Oncotarget. 2017; 8: 113598–613. doi: 10.18632/oncotarget.22748.

  7. Fei Y., Xiao L., Doetschman T. et al. Fibroblast growth factor 2 stimulation of osteoblast differentiation and bone formation is mediated by modulation of the Wnt signaling pathway. Journal of Biological Chemistry. 2011; 286(47): 40575–83. doi: 10.1074/jbc.M111.274910.

  8. Ito C., Mukudai Y., Itose M. et al. Tumor proteins D52 and D54 have opposite effects on the terminal differentiation of chondrocytes. BioMed Research International. 2017: 6014278. doi: 10.1155/2017/6014278.


About the Autors

Bulat I. Yalaev, 3-year post-graduate student of the laboratory of human molecular genetics at the Institute of biochemistry and genetics of Ufa Federal Research Center of the Russian Academy of Sciences. Address: 450054, Ufa, 71 Oktyabrya Avenue. Tel.: +7 (347) 235-60-88. E-mail: yalaev.bulat@yandex.ru
Anton V. Tyurin, PhD, associate professor, head of the Department of internal diseases of Bashkir State Medical University of the Ministry of Healthcare of Russia. Address: 450000, Ufa, 2 Shafiev Str. Tel.: +7 (347) 223-11-92. E-mail: anton.bgmu@gmail.com. ORCID: 0000-0002-0841-3024
Radik Z. Nurlygayanov, PhD, traumatologist-orthopedist of City clinical hospital No. 21. Address: 450071, Ufa, 3 Lesnoy Lane. Tel.: +7 (347) 232-32-88. E-mail: radiknur@list.ru
Rita I. Khusainova, doctor of biological sciences, associate professor, professor of the Department of medical genetics and fundamental medicine of Bashkir State Medical University of the Ministry of Healthcare of Russia, leading researcher of the laboratory of human molecular genetics at the Institute of biochemistry and genetics of Ufa Federal Research Center of the Russian Academy of Sciences. Address: 450054, Ufa, 71 Oktyabrya Avenue. Tel.: +7 (347) 235-60-88. E-mail: ritakh@mail.ru


Similar Articles

Back to Content

Бионика Медиа